A transcriptional regulator, IscR, of Burkholderia multivorans acts as both repressor and activator for transcription of iron-sulfur cluster-biosynthetic isc operon.
Identifieur interne : 000180 ( Main/Exploration ); précédent : 000179; suivant : 000181A transcriptional regulator, IscR, of Burkholderia multivorans acts as both repressor and activator for transcription of iron-sulfur cluster-biosynthetic isc operon.
Auteurs : Shouta Nonoyama [Japon] ; Kouhei Kishida [Japon] ; Keiichiro Sakai [Japon] ; Yuji Nagata [Japon] ; Yoshiyuki Ohtsubo [Japon] ; Masataka Tsuda [Japon]Source :
- Research in microbiology [ 1769-7123 ] ; 2020.
Abstract
Bacterial iron-sulfur (Fe-S) clusters are essential cofactors for many metabolic pathways, and Fe-S cluster-containing proteins (Fe-S proteins) regulate the expression of various important genes. However, biosynthesis of such clusters has remained unknown in genus Burkholderia. Here, we clarified that Burkholderia multivorans ATCC 17616 relies on the ISC system for the biosynthesis of Fe-S clusters, and that the biosynthetic genes are organized as an isc operon, whose first gene encodes IscR, a transcriptional regulatory Fe-S protein. Transcription of the isc operon was repressed and activated under iron-rich and -limiting conditions, respectively, and Fur, an iron-responsive global transcriptional regulator, was indicated to indirectly regulate the expression of isc operon. Further analysis using a ΔiscR mutant in combination with a constitutive expression system of IscR and its derivatives indicated transcriptional repression and activation of isc operon by holo- and apo-forms of IscR, respectively, through their binding to the sequences within an isc promoter-containing (Pisc) fragment. Biochemical analysis using the Pisc fragment suggested that the apo-IscR binding sequence differs from the holo-IscR binding sequence. The results obtained in this study revealed a unique regulatory system for the expression of the ATCC 17616 isc operon that has not been observed in other genera.
DOI: 10.1016/j.resmic.2020.06.005
PubMed: 32628999
Affiliations:
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Electronic address: nonoyma@ige.tohoku.ac.jp.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai, 980-8577</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author><author><name sortKey="Kishida, Kouhei" sort="Kishida, Kouhei" uniqKey="Kishida K" first="Kouhei" last="Kishida">Kouhei Kishida</name><affiliation wicri:level="4"><nlm:affiliation>Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai, 980-8577, Japan. 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Electronic address: yohtsubo@ige.tohoku.ac.jp.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai, 980-8577</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author><author><name sortKey="Tsuda, Masataka" sort="Tsuda, Masataka" uniqKey="Tsuda M" first="Masataka" last="Tsuda">Masataka Tsuda</name><affiliation wicri:level="4"><nlm:affiliation>Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai, 980-8577, Japan. Electronic address: mtsuda@ige.tohoku.ac.jp.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai, 980-8577</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author></analytic><series><title level="j">Research in microbiology</title><idno type="eISSN">1769-7123</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bacterial iron-sulfur (Fe-S) clusters are essential cofactors for many metabolic pathways, and Fe-S cluster-containing proteins (Fe-S proteins) regulate the expression of various important genes. However, biosynthesis of such clusters has remained unknown in genus Burkholderia. Here, we clarified that Burkholderia multivorans ATCC 17616 relies on the ISC system for the biosynthesis of Fe-S clusters, and that the biosynthetic genes are organized as an isc operon, whose first gene encodes IscR, a transcriptional regulatory Fe-S protein. Transcription of the isc operon was repressed and activated under iron-rich and -limiting conditions, respectively, and Fur, an iron-responsive global transcriptional regulator, was indicated to indirectly regulate the expression of isc operon. Further analysis using a ΔiscR mutant in combination with a constitutive expression system of IscR and its derivatives indicated transcriptional repression and activation of isc operon by holo- and apo-forms of IscR, respectively, through their binding to the sequences within an isc promoter-containing (Pisc) fragment. Biochemical analysis using the Pisc fragment suggested that the apo-IscR binding sequence differs from the holo-IscR binding sequence. The results obtained in this study revealed a unique regulatory system for the expression of the ATCC 17616 isc operon that has not been observed in other genera.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32628999</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1769-7123</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Jul</Month><Day>03</Day></PubDate></JournalIssue><Title>Research in microbiology</Title><ISOAbbreviation>Res Microbiol</ISOAbbreviation></Journal><ArticleTitle>A transcriptional regulator, IscR, of Burkholderia multivorans acts as both repressor and activator for transcription of iron-sulfur cluster-biosynthetic isc operon.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">S0923-2508(20)30059-0</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.resmic.2020.06.005</ELocationID><Abstract><AbstractText>Bacterial iron-sulfur (Fe-S) clusters are essential cofactors for many metabolic pathways, and Fe-S cluster-containing proteins (Fe-S proteins) regulate the expression of various important genes. However, biosynthesis of such clusters has remained unknown in genus Burkholderia. Here, we clarified that Burkholderia multivorans ATCC 17616 relies on the ISC system for the biosynthesis of Fe-S clusters, and that the biosynthetic genes are organized as an isc operon, whose first gene encodes IscR, a transcriptional regulatory Fe-S protein. Transcription of the isc operon was repressed and activated under iron-rich and -limiting conditions, respectively, and Fur, an iron-responsive global transcriptional regulator, was indicated to indirectly regulate the expression of isc operon. Further analysis using a ΔiscR mutant in combination with a constitutive expression system of IscR and its derivatives indicated transcriptional repression and activation of isc operon by holo- and apo-forms of IscR, respectively, through their binding to the sequences within an isc promoter-containing (Pisc) fragment. Biochemical analysis using the Pisc fragment suggested that the apo-IscR binding sequence differs from the holo-IscR binding sequence. The results obtained in this study revealed a unique regulatory system for the expression of the ATCC 17616 isc operon that has not been observed in other genera.</AbstractText><CopyrightInformation>Copyright © 2020 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nonoyama</LastName><ForeName>Shouta</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai, 980-8577, Japan. Electronic address: nonoyma@ige.tohoku.ac.jp.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kishida</LastName><ForeName>Kouhei</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai, 980-8577, Japan. Electronic address: kishida@ige.tohoku.ac.jp.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sakai</LastName><ForeName>Keiichiro</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai, 980-8577, Japan. Electronic address: k.sakai@ige.tohoku.ac.jp.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nagata</LastName><ForeName>Yuji</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai, 980-8577, Japan. Electronic address: aynaga@ige.tohoku.ac.jp.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ohtsubo</LastName><ForeName>Yoshiyuki</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai, 980-8577, Japan. Electronic address: yohtsubo@ige.tohoku.ac.jp.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tsuda</LastName><ForeName>Masataka</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai, 980-8577, Japan. Electronic address: mtsuda@ige.tohoku.ac.jp.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>07</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Res Microbiol</MedlineTA><NlmUniqueID>8907468</NlmUniqueID><ISSNLinking>0923-2508</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Burkholderia multivorans</Keyword><Keyword MajorTopicYN="N">Fe–S cluster</Keyword><Keyword MajorTopicYN="N">Iron</Keyword><Keyword MajorTopicYN="N">IscR</Keyword><Keyword MajorTopicYN="N">Transcriptional regulation</Keyword></KeywordList><CoiStatement>Declaration of Competing Interest The authors declare no conflicts of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>02</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>06</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32628999</ArticleId><ArticleId IdType="pii">S0923-2508(20)30059-0</ArticleId><ArticleId IdType="doi">10.1016/j.resmic.2020.06.005</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Région de Tōhoku</li></region><settlement><li>Sendai</li></settlement><orgName><li>Université du Tōhoku</li></orgName></list><tree><country name="Japon"><region name="Région de Tōhoku"><name sortKey="Nonoyama, Shouta" sort="Nonoyama, Shouta" uniqKey="Nonoyama S" first="Shouta" last="Nonoyama">Shouta Nonoyama</name></region><name sortKey="Kishida, Kouhei" sort="Kishida, Kouhei" uniqKey="Kishida K" first="Kouhei" last="Kishida">Kouhei Kishida</name><name sortKey="Nagata, Yuji" sort="Nagata, Yuji" uniqKey="Nagata Y" first="Yuji" last="Nagata">Yuji Nagata</name><name sortKey="Ohtsubo, Yoshiyuki" sort="Ohtsubo, Yoshiyuki" uniqKey="Ohtsubo Y" first="Yoshiyuki" last="Ohtsubo">Yoshiyuki Ohtsubo</name><name sortKey="Sakai, Keiichiro" sort="Sakai, Keiichiro" uniqKey="Sakai K" first="Keiichiro" last="Sakai">Keiichiro Sakai</name><name sortKey="Tsuda, Masataka" sort="Tsuda, Masataka" uniqKey="Tsuda M" first="Masataka" last="Tsuda">Masataka Tsuda</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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